HEPES (4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent. It is one of the twenty Good's buffers.

HEPES is commonly used as a biological buffering agent in biochemical research that helps maintain a stable pH in aqueous solutions. It is effective as a buffer, especially in the pH range …

Nov 10, 2025 · HEPES (4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a synthetic organic chemical buffering agent widely used across the life sciences to stabilize the pH of …

HEPES buffer is widely used across biological and biochemical research fields. Its primary use is in cell and tissue culture, where stable pH is important for cell viability, growth, and metabolism.

May 30, 2024 · HEPES, a very effective N-substituted aminosulfonic acid biological buffer, is an important flexible exfoliating component that reorganizes the stratum corneum and promotes …

Dec 17, 2025 · HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of …

Gibco HEPES (4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It is membrane impermeable, …

Researchers often prefer HEPES over traditional buffers like phosphate due to its superior performance in maintaining pH stability in a variety of applications, including molecular biology, …

HEPES is a zwitterionic buffer and is one of Good’s 12 buffers. It has a pKa value similar to its pH value, which makes it a strong pH stabilizer with a useful pH buffering range from 6.8 to 8.2.

HEPES, scientifically known as 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid, is a highly regarded zwitterionic organic chemical buffering agent. It plays a crucial role in numerous …