A major goal in biomedical research is to determine the mechanisms responsible for gene regulation. However, the promoters and operators that control transcription are often complex in nature, ...

ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis of extracellular vesicles (EVs), where removing excess antibodies ...

We have assessed the utility of RNA titration samples for evaluating microarray platform performance and the impact of different normalization methods on the results obtained. As part of the ...

This lab includes the complete titration procedure used to investigate how well a buffer resists changes in pH. Prepare a buffer solution by accurately calculating and measuring the required amounts ...

Epigenetics refers to the study of heritable changes that occur in gene expression. Such changes are induced by non-genetic mechanisms, without causing any change in the DNA sequence or gene structure ...

Complexes, such as protein/protein, nucleic acids/nucleic acid, protein/nucleic acid, protein/ligand, nucleic acid/ligand, are often difficult to crystallize even on well-defined biological systems, ...

I’m sure many of you reading this can remember your first acid-base titrations in chemistry class—the thrill of watching the pH indicator change color, and the fear of opening the stopper too much and ...

This lab includes a demonstration of the proper use of a burette system for titrations; including a detailed description, instructional video, and opportunity for students to collect data to ...